Monoclonal Antibodies in Drug Development

monoclonal antibodies antigen binding protein interaction

The global monoclonal antibody market has an annual value of USD 100 billion, boasting blockbuster drugs such as Humira and Keytruda. Monoclonal antibodies are immune cells that have the ability to bind to very specific targets. By doing so, they can elicit a response against virtually any disease. Today, monoclonal antibodies are among the most successful candidates for the development of new drugs. How do they work? And what makes them so successful?

Basics of Monoclonal Antibodies

Our immune system consists of immune cells, working day and night to keep our body healthy. It does this by taking out pathogens – stuff that the immune system thinks will make us ill – such as viruses and bacteria, among other foreign species. B cells play a big role in this, creating ‘tags’ that bind to pathogens, alerting the rest of the immune system to its presence. These ‘tags’ are known as antibodies.

In order for antibodies to recognize a pathogen, it has to contain a very specific binding region. It is this binding region that allows it to bind tightly to an epitope (also known as an antigen). An antigen can be a protein, a receptor, or even a whole virus; at any rate, antibodies detect antigens.

When our immune system is up and running normally, we do an excellent job producing a variety of unique antibodies. But what if we could design our own ‘tags’ to specifically target disease in the body? This idea in early 1980s inspired an entire field of research – monoclonal antibodies (mAbs).

monoclonal antibody production process mouse murine plasma hybridoma
By fusing an individual mouse immune cell with a tumor cell, we have a rapid production system for monoclonal antibodies.

In principle, this means that we design specific mAbs to target and destroy any pathogen in the body, without damaging the rest of our cells. Sounds great, doesn’t it? Unfortunately, since the approval of the first monoclonal antibody therapy in 1986, they haven’t quite hit ‘magic bullet’ heights.

Evolution of the Monoclonal Antibody

Now, the B cells that we clone to produce monoclonal antibodies usually come from mice; human cell lines are comparably less stable. Now, a life-threatening problem arises when we try to introduce these mAbs into our body. Our immune cells can sense this foreign material and proceed to attack it – the immune system can be quite silly sometimes.

To combat this, researchers quickly began to replace bits of the mouse antibody with human fragments, to discourage the immune cells from destroying it. It was a challenge of chopping mAbs up and putting them back together again, but this time, more human-like. However, these chimeric antibody therapies didn’t really take off, mainly due to adverse reactions and other side effects.

The real breakthroughs came about after humanized antibodies started to enter the field, in the early 1990s. These are essentially human antibodies, with the only mouse component being the antigen-binding regions. Other techniques – such as transgenic mammals and phage display – allow for the creation of fully human mAbs, ‘invisible’ to our immune system.

monoclonal antibody therapies approved FDA murine chimeric humanized mAbs
Murine and chimeric mAbs quickly made way for humanized and fully human ones1.

Other Shortcomings of mAbs

Another problem they face is poor bioavailability. As mAbs are ‘large’ molecules compared to the usual small molecule drugs, they are not easily absorbed into our bloodstream. If we simply ingest them, our liver can quickly metabolize them before they even get the chance to work. This means that their administration has to be direct – into the bloodstream – instead of the simple popping of a pill.

But other issues also plague the development of mAbs. Since they are produced in huge vats of cells as opposed to chemical reactors, it is difficult to optimize their conditions for growth. ‘Biologics’ – as they are called – also tend to have a wide range of impurities in the final product. Apart from the host cell’s proteins and DNA, growth factors and hormones must be removed from the final mixture. Some of these, like bacterial endotoxins, present risks of serious adverse reactions in patients. Technologies to aid the development of mAbs is, therefore, a huge field of research.

Related: Endotoxin Detection Using Horseshoe Crab Blood

Harnessing Their Potential

At the time of writing, there are 92 monoclonal antibody therapies on the market, with hundreds more in drug development pipelines. Now, we know that monoclonal antibodies offer us the ability to target and bind to specific antigens, causing therapeutic benefit. But how exactly does it do that? How does simple binding translate into mAbs being successful drugs?

Related: The Process and Costs of Drug Development

There are many ways that mAbs can be put to use, the obvious one being tests to detect the presence of certain substances. The most well-known use comes from its usefulness in enzyme-linked immunosorbent assays (ELISA); HIV detection and pregnancy test kits fall under this banner. In addition to diagnostics, mAbs binding can also alter inflammation and even cancer pathways. Since all processes in our body are governed by chemical binding, there is no limit to the therapeutic potential of mAbs! We’ll explore some below.

Humira (adalimumab)

The anti-inflammatory mAb Humira (adalimumab) was brought to market in 2002, for the treatment of rheumatoid arthritis. It works by binding and inhibiting tumor necrosis factor-alpha (TNFα), a key protein in the immune response. Patients suffering from rheumatoid arthritis usually have high TNFα levels, causing the immune system to attack their joints2. This painful autoimmune disease has no known cure, affecting tens of millions of adults worldwide.

humira adalimumab autoinjector pen box
Humira autoinjector pen for subcutaneous injection.

Since then, Humira has been approved to further treat a wide range of autoimmune diseases. Despite its patent expiry, Humira maintains its superstar status, with Abbvie reporting sales of over USD 18 billion in 2017.

Keytruda (pembrolizumab)

Perhaps unsurprisingly, oncology is another key target for mAbs. Cancer cells tend to have certain proteins on their surfaces which bind to and inhibit the action of immune cells. If we can block this interaction, then the immune system can perform the dirty work of destroying the tumor. This is the mechanism behind Merck and Co.’s Keytruda (pembrolizumab).

Our immune cells possess programmed cell death protein 1 (PD-1) receptors, that serve to block immune action when bound. This is known as an immune checkpoint – a system that prevents the immune system from accidentally targeting them3. However, certain cancers cells take advantage of this by overproducing proteins that bind to PD-1, stopping immune cells from killing them.

keytruda pembrolizumab 5DK3 structure
Keytruda (pembrolizumab), a humanized monoclonal antibody.

Keytruda binds to and blocks the PD-1 receptor, preventing cancer cells from binding4. This allows the immune system to recognize the tumor as foreign, and initiate a response against it. Although Keytruda’s initial approval was for the treatment of melanomas, it is now indicated for other cancers that have certain genetic anomalies.

Future Drug Discovery

The specific and high-affinity binding of mAbs, compared to traditional small molecule therapies, make them attractive targets for drug discovery. Combine this with ever-advancing technologies for their development and production, and there is no doubt that monoclonal antibody therapies will continue to prosper.

In an ideal world, we will be able to screen targets for their unique epitopes, and efficiently design monoclonal antibodies that bind with high affinity to them. Through the regulation of receptors and signal pathways, virtually any disease has a direct cure. Perhaps Paul Erhlich’s magic bullet may not be too far off the mark.

Cover graphic: artistic rendition of parent B cells producing monoclonal antibodies by Melanie (@nanoclustering)

Reference

  1. Reichert, J. M. (2001). Monoclonal antibodies in the clinic. Nature Biotechnology19(9), 819.
  2. Jongen-Lavrencic, M., Peeters, H. R., Wognum, A., Vreugdenhil, G., Breedveld, F. C., & Swaak, A. J. (1997). Elevated levels of inflammatory cytokines in bone marrow of patients with rheumatoid arthritis and anemia of chronic disease. The Journal of rheumatology24(8), 1504-1509.
  3. Francisco, L. M., Sage, P. T., & Sharpe, A. H. (2010). The PD‐1 pathway in tolerance and autoimmunity. Immunological reviews236(1), 219-242.
  4. Le, D. T., Durham, J. N., Smith, K. N., Wang, H., Bartlett, B. R., Aulakh, L. K., … & Wong, F. (2017). Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade. Science357(6349), 409-413.

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