Category: University Level Guides

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A Guide To Quantitative Polymerase Chain Reaction (qPCR)

qPCR – also known as Real-Time Polymerase Chain Reaction – is currently the gold standard for detection and quantification of RNA in a small number of tissues. However, the technique itself depends on a range of factors; let’s take a look at some tips and tricks to increase your chances of success.

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How To: Decide Between Liquid or Gas Chromatography

Chromatography is a technique used by scientists throughout all disciplines; many different separation techniques exist, each catered to a specific type of analyte. That’s because the principle behind it is simple, separate a mixture of compounds based on their physical or chemical properties. These techniques can be very broadly classified by their ‘mobile phase’, either liquid or gas.

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How To: Statistics – ANOVA

One thing that regularly stumps scientists is the handling of data. We seem to be very good at generating obscene amounts of it, but representing it meaningfully can be a little off putting if you don’t happen to be a bioinformatician. In previous tutorials we looked at hypothesis testing using variations of the t-Test, and we continue the series by comparing more than 2 samples sets with ANOVA.

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